PARTIAL PURIFICATION OF THE ORGANISM OF PSITTACOSIS GROWN IN CHICK EMBRYO YOLK SAC

Abstract

A procedure described for the partial purification of suspensions of the organism of psittacosis utilizes tryptic digestion, calcium chloridedextran sulfate precipation, and centrifugation through a sucrose barrier. More than 50% of the organisms in the original suspension are recovered in 80% of the original volume with approximately 95% of the lipids and 85% of the proteins removed. This method is suggested as a preliminary purification procedure in laboratories desiring relatively large amounts of purified agent for studies on metabolism, physical properties, or antigenicity of the organism. Significant aspects of the investigations leading to the selection of the procedure are included.

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Document Details

Document Type
Technical Report
Publication Date
Dec 01, 1964
Accession Number
AD0455886

Entities

People

  • Warren G. Dorsey
  • William C. Patrick Iii.
  • William N. Shirey

Organizations

  • United States Army Biological Warfare Laboratories

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Biological Laboratories
  • Biomedical Research
  • Calcium
  • Calcium Compounds
  • Chlorides
  • Ethers
  • Government Procurement
  • Governments
  • Gram-Negative Bacterial Infections
  • Laboratory Animals
  • Lipids
  • Materials
  • Physical Properties
  • Proteins
  • Sodium
  • Sodium Compounds
  • United States

Fields of Study

  • Biology

Readers

  • Microbial Pathology
  • Systems Analysis and Design
  • Virology (or Medical Virology).