CORTICOSTEROID EFFECT ON AMINO ACID INCORPORATION BY RAT LIVER RIBONUCLEOPROTEIN PARTICLES,
Abstract
The incorporation of the 14C label from leucine14C into protein is catalyzed by ribonucleoprotein particles (RNP) prepared from rat liver cells. The results obtained have demonstrated that, in the incubation system used, RNP-particles prepared from livers of normal and adrenalectomized rats incorporated 14C-labeled amino acid at a rate proportional to the length of time of incubation. When RNP-particles from livers of adrenalectomized rats are used, the 14C-amino acid incorporated into the protein is more than twice that with RNP-particles from normal rats. However, if adrenalectomized rats are injected with corticosterone and the RNP-particles from livers of these animals are added to the incubation system, the increased amino acid incorporation is eliminated. Preliminary electrophoretic separation of the labeled protein resulted in two protein peaks, the first representing 14% and the other 86% of the total protein, with the radioactivity associated entirely with the first protein peak. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 1964
- Accession Number
- AD0459090
Entities
People
- Frankc DeVenuto
- Robert J. G. Lange
Organizations
- United States Army Medical Research Laboratory