ATTENUATED TOTAL REFLECTANCE IN INFRARED SPECTROPHOTOMETRY OF BIOLOGICAL SYSTEMS

Abstract

The attenuated total reflectance (ATR) method for obtaining infrared absorption spectra has been shown to be a feasible technique for the study of biological and other materials in their normal aqueous environment. Water-compensated ATR spectra for a number of simple compounds, a protein (bovine serum albumin), and a bacterium (Serratia marcescens) in water solution or suspension, were generally similar to normal transmission spectra for dried samples; but showed some distinctive features not present in the latter. Some limitations of the method and steps taken to overcome them are discussed.

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Document Details

Document Type
Technical Report
Publication Date
Mar 01, 1965
Accession Number
AD0459388

Entities

People

  • Edward J. Weneck
  • Jack Wagman

Organizations

  • United States Army Biological Warfare Laboratories

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Absorption
  • Albumins
  • Aqueous Solutions
  • Biological Laboratories
  • Films
  • Health Services
  • Infrared Spectra
  • Maryland
  • Materials
  • New Jersey
  • New York
  • Public Health
  • Reflectance
  • Refractive Index
  • Spectra
  • Systems Biology
  • United States

Fields of Study

  • Physics

Readers

  • Image Processing and Computer Vision.
  • Microbial Pathology
  • Molecular and Cellular Biochemistry