EFFECT OF ENZYMATIC FUNCTIONAL GROUP INHIBITORS ON PROTEOLYTIC ENZYMES OF CROTALUS ATROX VENOM
Abstract
Crotalus atrox venom proteases are inhibited by several functional group specific inhibitors. Heavy metal ions inhibit, whereas calcium and other divalent alkaline earths have a general activating effect on these proteases. The type and number of proteases in crude venom can be discerned by combined use of these reagents and four different substrates. Two of these substrates, N- benzoyl-DL-arginine-p-nitroanilide and congocoll have recently been introduced as substrates for trypsin, but have not as yet been used to assay proteolytic enzyme activity of snake venoms.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 05, 1965
- Accession Number
- AD0464196
Entities
People
- John H. Brown
Organizations
- United States Army Medical Research Laboratory