CONTROLLED PH AND OXIDATION-REDUCTION POTENTIAL WITH A NEW GLASS TISSUE CULTURE FERMENTOR

Abstract

A 1-liter fermentor was designed and tested for use as a tissue-culture vessel. It features a temperature control device; impeller agitation without the necessity of a shaft seal; and a means for measuring, recording, and controlling both pH and oxidation-reduction potential (ORP). Tests have shown the ORP to change fairly rapidly with impeller speed variations under conditions of a continuous carbon dioxide - air overlay. Working with strain L mouse fibroblasts (Earle), cell counts of more than 1.25 x 10 to the 6th power per milliliter without centrifugation and medium renewal were achieved and cell counts were maintained above 1 x 10 to the 6th power for more than 30 hours. With the vessel studied, pH control was =0.05, the ORP control was =10 millivolts. Controlled environments for tissue cell metabolic studies are entirely feasible with this system. (Author)

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Document Details

Document Type
Technical Report
Publication Date
May 01, 1965
Accession Number
AD0464214

Entities

People

  • David A. Parker
  • Louis E. Schneider
  • Roger W. Johnson
  • William F. Daniels

Organizations

  • United States Army Biological Warfare Laboratories

Tags

Communities of Interest

  • Biomedical
  • Cyber
  • Ground and Sea Platforms

DTIC Thesaurus Topics

  • Accuracy
  • Amino Acids
  • Assembly
  • Ball Bearings
  • Cell Count
  • Chemistry
  • Control Panels
  • Control Systems
  • Environment
  • Health Services
  • Materials
  • Plastic Explosives
  • Public Health
  • Stainless Steel
  • Tissue Culture
  • United States
  • Vitamin C

Readers

  • Cardiovascular Physiology
  • Mathematics or Statistics
  • Virology (or Medical Virology).