STUDIES IN RED CELL PRESERVATION.
Abstract
Electrophoretic studies show that tri-, tetra-, and hexaphosphates form complexes with hemoglobin. The absolute and percentage values for Component B and organic phosphate from the same hemolysate of stored ACD and CPD blood supplemented with adenine, adenosine, inosine, and mixtures of these compounds were determined. The regression lines would approach the zero intercept if the high values for Component B and organic phosphate were not included. Nevertheless, the correlation coefficients are high for anaerobically and aerobically stored bloods. The organic phosphate compounds in the washed red cells of bloods supplemented with adenine, adenosine, or inosine and stored at 4 C for 6 weeks were determined at 2-week intervals by anion exchange analysis. These bloods were collected in ACD and CPD and stored anaerobically and aerobically. Under aerobic conditions, the pH values are greater. The highest diphosphoglycerate (DPG) values are observed in the adenosine-supplemented bloods, particularly wh n the pH is high. The concentrations of ATP and adenine are greatest in ACD bloods particularly when supplemented with adenine. No consistent relationship exists between pH and DPG or ATP concentrations. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 30, 1965
- Accession Number
- AD0465913
Entities
People
- Alfred Chanutin
Organizations
- University of Virginia