COULTER COUNTER THEORY AND COINCIDENCE COUNT CORRECTIONS: MODIFICATIONS AND ADDITIONS FOR WORK WITH SPHERES ABOUT ONE MICRON IN DIAMETER
Abstract
This paper deals with the use of the Coulter Counter to count and size particles whose number average volume is between 0.33 cubic micron and 2.5 cubic micron. It was our purpose to determine whether particles of this size could be accurately counted and sized and to determine whether the existing theory could be applied directly or modified to apply to suspensions of such particles. It has been shown that the implicit assumption of negligible surface conductance of polystyrene particles is acceptable. The device has proved capable of enumerating particles whose volume is at least as small as 0.33 cubic micron, but accurate sizing, employing the volume-threshold relationship developed, is limited to particles whose volume lies between 0.75 cubic micron and 2.5 cubic micron. Establishment of empirical curves for each combination of controlled variables will probably permit accurate sizing of particles smaller than 0.75 cubic micron, the volume of many commonly studied bacteria.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 01, 1965
- Accession Number
- AD0465915
Entities
People
- William B. Mercer
Organizations
- United States Army Biological Warfare Laboratories