NATURAL RESISTANCE OF HAMSTER CELLS TO 8-AZAGUANINE
Abstract
In studies designed to introduce mutations into selected animal cell lines in culture, cell lines derived from the Syrian hamster were found to be naturally resistant to 8-azaguanine. Resistance to the drug was measured by plating colony-forming units at different drug concentrations and determining the concentration at which 50% of the colony-forming units formed colonies. By this technique it was shown that KB cells (human) and L cells (mouse have approximately the same susceptibility to 8-azaguanine as reported by others for L, D98 (human), and P388 (mouse). On the other hand, both P113, a polyoma- transformed derivative of embryonic hamster kidney, and BHK21C13, an untransformed hamster cell displaying contact inhibition, proved 50 times more resistant than the cell lines of human and murine origin. The higher resistance of the hamster cells to 8-azaguanine is specific, since these cells are still susceptible to the unrelated drug 5-bromodeoxyuridine. Preliminary results suggest that the basis for natural resistance is not loss of inosinic-guanylic acid pyrophosphorylase activity, because the cells form colonies under conditions in which growth depends on an exogenous supply of hypoxanthine (HAT medium). The naturally resistant hamster cells therefore resemble the mutant D98/AG described by Szybalski.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1965
- Accession Number
- AD0468160
Entities
People
- Alan Richter
Organizations
- United States Army Biological Warfare Laboratories