IRON METABOLISM AND TRYPTOPHAN PYRROLASE ACTIVITY IN ENDOTOXIN-POISONED AND CORTISONE-PROTECTED MICE
Abstract
The hypoferremia which follows the intraperitoneal injection of mice with heat-killed Salmonella typhimurium is not mitigated by protection with cortisone given simultaneously. A single injection of cortisone causes a transient hyperferremia. None of these changes in concentration of plasma iron occurs when mice are maintained in a 5 C environment during experimental treatment. The alteration in iron metabolism during endointoxication does not seem to affect the availability of the iron-porphyrin coenzyme of tryptophan pyrrolase. An initial response of mice to injections of endotoxin or cortisone or both is a translocation of hematin, and the availability of hematin permits an increase in tryptophan pyrrolase activity before the stress-mediated induction of the enzyme becomes apparent. The increase in available activator, with the subsequent decrease in amount of normally inactive apoenzyme, occurs at the same rate and to the same extent in mice housed at 5 C and 25 C. It is suggested that the increased demand for an iron-containing substance in the liver during stress contributes to the accompanying hypoferremia.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 1965
- Accession Number
- AD0469244
Entities
People
- George N. Eaves
- L. J. Berry
Organizations
- Bryn Mawr College