HEMAGGLUTINATION-INHIBITION METHOD AND IMMUNOFLUORESCENCE STAINING WITH VEE VIRUS
Abstract
Hemagglutination and fluorescent antibody are compared for the direct detection of virus devoid of host cells. The minimum number of tissue plaque- forming units of Venezuelan equine encephalomyelitis virus that could be detected by the hemagglutination technique was determined. Similar concentrations of the virus in bovine albumin borate saline, brain-heart infusion broth, and demineralized water were tested by the fluorescent antibody technique. Somewhat higher concentrations of the virus in bovine albumin borate saline were used in the hemagglutination-inhibition test. The quantitative hemagglutination procedure employed for these studies was carried out at 37 C for 75 minutes with variations in concentration of goose red cells. As a result of lowering the red cell concentration, smaller concentrations of virus were detected. The direct fluorescent antibody staining procedure applied to slide preparations containing known numbers of tissue culture plaque-forming units of virus was negative. Adsorbed viral antigen on agglutinated goose erythrocytes was visualized by direct and indirect FA techniques.
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 1965
- Accession Number
- AD0469384
Entities
People
- Michael Shepel
Organizations
- United States Army Biological Warfare Laboratories