A UNIFIED APPROACH TO THE ANALYSIS OF HUMAN SERUM LIPIDS FOR CLINICAL INVESTIGATION.

Abstract

A method is described by which the organic solvent-soluble components of 1 ml. of serum are extracted and purified. The extract is dried and weighed to determine the total lipid content. The dried extract is then redissolved in petroleum ether and pipetted onto a prepared column of silicic acid from which the four major components (cholesteryl esters, nonesterified cholesterol, glycerides, and phospholipids) are eluted separately and are quantitated by spectrophotometric methods. Detailed procedures are given for each step in the analysis. (Author)

Document Details

Document Type
Technical Report
Publication Date
Sep 01, 1965
Accession Number
AD0474253

Entities

People

  • Dorothy F. Wease

Organizations

  • United States Air Force School of Aerospace Medicine

Tags

DTIC Thesaurus Topics

  • Alkanes
  • Biomolecules
  • Chemical Compounds
  • Cholesterol
  • Cholesteryl Esters
  • Esters
  • Glycerides
  • Lipids
  • Membrane Lipids
  • Organic Solvents
  • Petroleum
  • Solvents

Readers

  • Analytical Chemistry