ASCORBIC ACID PROTECTION AGAINST INACTIVATION OF LYSOZYME AND ALDOLASE BY IONIZING RADIATION.
Abstract
Solutions of lysozyme and aldolase (1% and 0.5%) were irradiated with Co60 gamma rays in the presence of different concentrations of ascorbic acid. This agent protected the enzymes even at 0.0005 M concentration. The protection was not quite as good as that obtained with GED when compared mole for mole, but was better than that obtained with dehydroascorbic acid. Radiation chemistry studies with C14-labeled ascorbic acid in enzyme solutions showed oxidation of ascorbic acid and binding to the enzymes. These results are interpreted in terms of mechanism of protective action. Comprehensive testing of ascorbic acid as a radiation-protective agent in animals is suggested. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 01, 1965
- Accession Number
- AD0474471
Entities
People
- Bernard Shapiro
- George Kollmann
- Joel N. Asnen
Organizations
- Jefferson Einstein Hospital