NATURAL DRUG RESISTANCE OF HETEROGENEOUS SINGLE CELL TRANSPLANTS

Abstract

Population analysis of a cancer clone derived from a single cancer cell was made, using a clonal transplant-strain of the rat ascites hepatoma, CL- 1-AH-66F. The natural resistance to nitrogen mustard N-oxide and the chromosomal constitution were studied, after establishing subclonal tumors from the original clone CL-1-AH-66F. The results indicated the following facts: (1) The cancer clone is not a uniform but a complex population of cells with different degrees of drug resistance. (2) The degree of drug resistance of the original clone and its subclones varies naturally within a certain range during serial passages of them in compatible hosts. (3) No specific correlation was found between the degree of drug resistance and chromosomal feature of the subclones. (4) It was suggested that the variation in drug resistance of cancer clone is not always associated to the genetic heterogeneity of cells in the cancer clone.

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Document Details

Document Type
Technical Report
Publication Date
Sep 29, 1965
Accession Number
AD0474986

Entities

People

  • Tomizo Yoshida

Tags

DTIC Thesaurus Topics

  • Abstracts
  • Cells
  • Chromosomes
  • Demographic Cohorts
  • Diploid Cells
  • Drug Resistance
  • Elements
  • Far East
  • Government Procurement
  • Governments
  • Materials
  • Military Research
  • National Security
  • Nitrogen
  • Nitrogen Mustards
  • Transplants
  • United States

Fields of Study

  • Biology

Readers

  • Analytical Chemistry
  • Immunology
  • Molecular Biology and Genetics

Technology Areas

  • Biotechnology