MICROBIAL UTILIZATION OF HYDROCARBON FUEL FORMULATIONS WITH THE PRODUCTION OF GUMS, SLIMES, SLUDGE AND SURFACE ACTIVE COMPOUNDS
Abstract
The role of microorganisms in the deterioration of fuels and in the production of sludges and surfactants was investigated. RP-1 and CITE (compression Ignition Turbine Engine) fuels supported microbial growth in 10% natural sea water medium. Growth was stimulated by three approved fuel soluble corrosion inhibitors and by the addition of inorganic nitrogen and phosphorus salts to the aqueous medium. However, fuel exposed to microorganisms for 16 weeks still met military specifications for quality. The type of hydrocarbon fuel component which supported growth of eight pure and mixed cultures of bacteria and fungi was established. These cultures were then used to produce sludge from fuel and pure hydrocarbons. Methods were outlined for the fractionation and analysis of sludge. Insoluble sludge was found to be primarily microbial cells. Several solvent soluble fractions were characterized as normal cell components and fuel components oxidized by the test organisms. No substantial accumulations of extracellular proteins and carbohydrates were observed. Metabolic studies indicated that recognized pathways were followed for the oxidation of n-paraffins and naphthalene.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 01, 1965
- Accession Number
- AD0476174
Entities
People
- A. R. Rizzuto
- A. T. Knecht Jr.
- D. J. Muhlbaier
- J. W. Kools