EFFECT OF FORMALIN ON THE IMMUNOCHEMICAL AND BIOLOGICAL ACTIVITY OF STAPHYLOCOCCAL ENTEROTOXIN
Abstract
Purified staphylococcal enterotoxin B was treated with formalin at 37 C and pH 7.3. The effect of formalin concentration and duration of exposure was studied by immunochemical and biological means. By 8 hours, toxin treated with 0.15 or 0.30% formalin (v/v) lost some of its ability to precipitate antibody. At 48 hours the toxoid behaved as a cross-reacting antigen in that an antibody excess occurred throughout the range of the quantitative precipitin titrations and the zone of antibody excess was followed by a zone in which both antigen and antibody were in excess. In the Ouchterlony double gel diffusion test after 48 hours, toxoid gave a reaction of partial identity when compared with toxin. When tested by immunoelectrophoresis at pH 6.0, 0.1 M PO4 buffer, the formalin- treated toxin migrated further toward the cathode than did the toxin.
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 01, 1965
- Accession Number
- AD0476396
Entities
People
- David A. Espeseth
- Edward J. Schantz
- Sidney J. Silverman
- William G. Roessler
Organizations
- United States Army Biological Warfare Laboratories