DISTRIBUTION OF LYMPHATIC TISSUE AND BETA-GALACTOSIDASE-POSITIVE CELLS IN THE SPLEEN OF NEW ZEALAND RABBITS
Abstract
New Zealand rabbits were injected with 7S human gamma-globulin, fraction II human gamma-globulin, and isopropyl thiogalactoside and sacrificed at 4, 24, 48, 72, 96 hours and 7 days. Marginal and central zones of lymphoid follicles measured 0.15227 plus or minus 0.012 and 0.08527 plus or minus 0.004 sq.mm. The 7S gamma-globulin showed changes in marginal zone but only in 48 hours and 7 days in the central zone. Fraction II showed no changes. The number of polymorphonuclear leukocytes with 7S gamma-globulin showed marked increase but none with isopropyl-beta-D-thiogalactopyranoside. There were no changes in reticular cells known to be beta-galactosidase-positive. The 5-bromo-4 chloroindol-3-yi-beta-D-galactopyranoside was used to demonstrate enzyme. Changes were instituted in substrate that enabled us to study enzyme in 2- microns sections at short incubation time, increasing the sensitivity of the reaction. The enzyme was distributed in reticular cells around central zone, trabeculae, and cortical sinuses. Enzyme changes apparently were not associated with cellular changes.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1966
- Accession Number
- AD0476474
Entities
People
- Alfred C. Standen
- Bjarne Pearson
Organizations
- United States Army Biological Warfare Laboratories