REGULATION OF METABOLISM IN WORKING MUSCLE IN VIVO. I. CONCENTRATIONS OF SOME GLYCOLYTIC, TRICARBOXYLIC ACID CYCLE, AND AMINO ACID INTERMEDIATES IN INSECT FLIGHT MUSCLE DURING FLIGHT.
Abstract
The concentrations of 28 metabolites were measured concurrently in the flight muscle of the blowfly (Phormia regina) after flight periods ranging from 5 sec to 1 hr. Measurements were taken at flight initiation and during continuous flight. Coincident-change measurements identical to the 100-fold increase in glycolytic flux at the initiation of flight provide a unique system to determine the steps that control in vivo glycolysis in transition from a resting tissue to one performing strenuous work. The sites of regulation demonstrated in this system were (1) the phosphorolysis of fructose-6-P, (2) the cleavage of trehalose, and (3) the phosphorolysis of glycogen. Additional controls at alpha-glycero-P and proline oxidation are postulated. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 01, 1965
- Accession Number
- AD0477587
Entities
People
- Bertram Sacktor
- Eva Wormser-shavit
Organizations
- Edgewood Chemical Biological Center