INHIBITION OF PROTEIN SYNTHESIS BY SPERMINE IN GROWING CELLS OF STAPHYLOCOCCUS AUREUS
Abstract
Staphylococcus aureus SFL 9725 incorporated valine-C14 into cellular protein. This incorporation was inhibited by spermine when the pH of the culture was, adjusted to 7.8 and the inhibition was antagonized partially by Mg(++). The incorporation of C14 labeled leucine, phenylalanine, lysine, arginine, and possibly glutamic acid was inhibited to a much greater extent than that of alanine, glycine, or threonine. The uptake of spermine-C14 by S. aureus cells was rapid. More than 50% of the radioactivity resided in the soluble extract. The protein fraction of the soluble extract contained 99% of the label, whereas the ribonucleic acid and deoxyribonucleic acid fractiosn contained little or no spermine-C14.
Document Details
- Document Type
- Technical Report
- Publication Date
- Feb 01, 1966
- Accession Number
- AD0479136
Entities
People
- Mischa E. Friedman
- Uriel Bachrach