PURIFICATION OF HISTAMINE SENSITIZING FACTOR OF BORDETELLA PERTUSSIS

Abstract

Purification of histamine sensitizing factor (HSF) of Bordetella pertussis was attempted from two sources: 1. e. sonicate of the bacterial cells and the culture supernatant. Subcellular fractions were prepared from the cells disintegrated by sonic oscillation. A fraction sedimentable at 105,000 x g for 90 minutes contained potent HSF activity as well as lethal toxicity. This fraction was mainly composed of ribosome particle. Electrophoretic experiments and ultracentrifugal analysis of the ribosomal fraction digested with protease or ribonuclease indicated that HSF activity can not be referred to the ribosome particle itself. HSF could be extracted from the ribosomal fraction with surface active agents such as sodium deoxycholate. Lethal toxicity was completely disappeared by this treatment. Several methods for purification of HSF from the culture supernatant, such as density gradient electrophoresis, pH gradient elution and polyvinyl pyrrolidone treatment, were surveyed.

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Document Details

Document Type
Technical Report
Publication Date
Sep 07, 1965
Accession Number
AD0479965

Entities

People

  • Yoshio Kuwajima

Organizations

  • Osaka City University

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Bacterial Toxins
  • Cells
  • Cellular Structures
  • Chemical Analysis
  • Classification
  • Contracts
  • Culture Media
  • Electrophoresis
  • Enzymes
  • Histamine
  • Materials
  • Organelles
  • Particles
  • Polyvinyls
  • Security
  • Subcellular Fractions
  • Toxicity

Fields of Study

  • Biology

Readers

  • Immunology
  • Molecular and Cellular Biochemistry