DIFFERENCES IN PROTEIN CONTENTS AMONG FIBROBLAST CELL LINES GROWN IN MONOLAYER CULTURES
Abstract
The changes in cell protein values of cat kidney and McCoy's human synovial cell lines were determined during various phases of monolayer growth in a lactalbumin hydrolyzate - 10% calf serum medium. The McCoy's cell protein values ranged from 1/230 000 000 to 1/574 000 000 mug/cel whereas the cat kidney cell values ranged from 1/131 000 000 to 1/367 000 000 mug/cell. Maximum values for each cell line were recorded during a short period in the lag phase of growth. Essentially constant minimum values were observed after the cultures reached a slowly proliferating, densely populated, post-confluent state. For this reason, the characteristic protein values reported for each cell line were determined during the latter part of the logarithmic growth phase.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 01, 1966
- Accession Number
- AD0481027
Entities
People
- Kiyoshi Higuchi
- Raymond R. Galacci