REPLICATION OF RIFT VALLEY FEVER VIRUS IN L CELL SUSPENSION CULTURES
Abstract
It has been possible to propagate Rift Valley fever (RVF) virus and obtain high virus yields in L cell suspension cultures maintained in a chemically defined medium. Peak titers (10 to the 8th power to 10 to the 8.5 power plaque-forming units/ml) were obtained in 24 hours and were comparable to plaque titers obtained in monolayer cultures propagated in a serum-containing medium. The addition of stabilizing compounds (bovine albumin and protamine sulfate) at the time of inoculation resulted in increased in vivo titers but no increase was observed in the plaque titers. The addition of serum to the samples prior to storage enchanced the stability of the virus. Substitution of fructose for glucose in the defined medium reduced the virus yields. However, the addition of proline and serine to the fructose-containing medium restored the virus yields to the level obtained with the glucose medium.
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 1966
- Accession Number
- AD0481043
Entities
People
- Henry R. Tribble Jr.
- John J. Boyle
Organizations
- United States Army Biological Warfare Laboratories