ELECTRON MICROSCOPIC AND IMMUNOFLUORESCENT STUDY OF CHIKUNGUNYA VIRUS INFECTION IN TISSUE CULTURE

Abstract

The development of chikungunya virus was investigated by immunofluorescent staining and electron microscopic observations. The virus was propagated in hamster kidney cells and viral antigen was first detected by immunofluorescence at 8 hours. The first alteration in subcellular structure, however, was observed at 12 hours. At that time electron-dense particles were seen surrounding cytoplasmic vacuoles. Mature virus particles were seen only within cytoplasmic vacuoles or in juxtaposition to the external surface of the cytoplasmic membrane. These particles were similar morphologically to particles seen in partially purified brain suspension from infected suckling mice. The results are compared with those reported in the literature for other arboviruses.

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Document Details

Document Type
Technical Report
Publication Date
Feb 01, 1966
Accession Number
AD0481044

Entities

People

  • John D. White
  • Lazarus L. Kupferberg

Organizations

  • United States Army Biological Warfare Laboratories

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Antigens
  • Arbovirus Infections
  • Arthropod-Borne Encephalitis
  • Biological Laboratories
  • Cells
  • Cytoplasm
  • Electron Microscopy
  • Encephalitis
  • Endoplasmic Reticulum
  • Infection
  • Microscopy
  • Tissue Culture
  • Tissues
  • Virion
  • Viruses
  • Wound Infections
  • Yellow Fever

Fields of Study

  • Biology

Readers

  • Molecular and Cellular Biochemistry
  • Plasma Physics / Magnetohydrodynamics
  • Virology (or Medical Virology).

Technology Areas

  • Microelectronics