ASSAY OF COXIELLA BURNETII BY ENUMERATION OF IMMUNOFLUORESCENT INFECTED CELLS
Abstract
A method for quantitative assay of C. burnetii was developed based on immunofluorescent staining of infected L cell monolayers and enumeration of cells containing fluorescent rickettsiae within 48 hours after inoculation. Adsorption of rickettsiae onto coverslip cell cultures was accelerated and highly efficient when augmented by centrifugal force. By this procedure, a proportionality was demonstrated between the number of infected cells and the volume of inoculum. The incubation period of 48 hours for inoculated cell cultures was established from sequential observations of the development of rickettsial infection of cells and from counts of infected cells. The relationship between rickettsial concentration and cell-infecting units of rickettsiae was linear; the distribution of infected cells was random. Compared with yolk sac inoculation of chick embryonated eggs, the infected cell-counting assay of C. burnetii was more rapid, precise, and sensitive.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 01, 1966
- Accession Number
- AD0482256
Entities
People
- Kenneth O. Cooke
- Nicholas Hahon