INTERSPECIES TRANSFORMATION BETWEEN BACILLUS SUBTILIS AND BACILLUS LICHENIFORMIS,
Abstract
Interspecies transformation was obtained between Bacillus subtilis and Bacillus licheniformis for the streptomycin resistance marker in both directions. A comparison of the reciprocal crosses indicates at least a 2-log difference in efficiency of transformation, depending on the direction of the cross. With B, licheniformis as the recipient and B, subtilis as the source of heterologous DNA, the number of interspecific transformants was 1/10,000 times the number of intraspecific transformants obtained with homologous DNA. When B, licheniformis DNA was used to transform B, subtilis number of interspecific transformants was only 1/10 to 1/100 times the number of intraspecific transformants. This work supports the existence of a conserved core of genetic information among the members of the genus Bacillus. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Apr 01, 1966
- Accession Number
- AD0482510
Entities
People
- Curtis B. Thorne
- Darrel D. Gwinn
- Ivan D. Goldgerg