SOME CHARACTERISTICS OF PLAQUE VARIANTS OF RIFT VALLEY FEVER VIRUS
Abstract
Two variants of Rift Valley fever virus have been isolated that differ from each other in plaque size. The biological characteristics of these isolates have been investigated in vivo and in vitro. The size of both variants increased when DEAE dextran was added to the agar overlay. The plaque size of both variants was equally reduced by low concentrations of sodium bicarbonate in the overlay, although the infectivity was not affected. Compared with the large- plaque virus, the small-plaque isolate was more readily adsorbed, more stable at 56 C, grew faster, and had a higher virus yield from monolayers of mouse fibroblast cells. The large-plaque virus required 100 times more plaque-forming units to kill mice by the intraperitoneal route of inoculation than the small- plaque, and also produced a delay in death of the inoculated mice.
Document Details
- Document Type
- Technical Report
- Publication Date
- May 01, 1966
- Accession Number
- AD0482585
Entities
People
- John J. Boyle