A SERUM-FREE AUTOCLAVED MEDIUM FOR GROWTH OF ANIMAL CELLS IN SUSPENSION
Abstract
A medium, sterilizable by autoclaving, was formulated for growth of animal cells in suspension culture. The medium contains peptone, proteose peptone, glutamine, and cysteine plus the salts and glucose described by Nagle for defined media. Methylcellulose is not required. Populations of HeLa, cat kidney, and L cells 4,100,000, 2,000,000 and 2,200,000 per ml were obtained after 22, 12, and 14 days of incubation, respectively. The medium provides a basis for studies leading to a heat-stable chemically defined medium. In its present form it is an easily prepared bacteriological-type medium with many possible applications in monolayer and suspended cell culture.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 01, 1966
- Accession Number
- AD0482602
Entities
People
- Stanley C. Nagle Jr.