IN VITRO INTERACTIONS BETWEEN RABBIT ALVEOLAR MACROPHAGES AND PASTEURELLA TULARENSIS

Abstract

Rabbit alveolar macrophages were successfully employed in a study of host cell - Pasteruella tularensis interactions in vitro. Under cell culture conditions in which inhibitory antibiotics were not employed and small infection ratios were used, the relative in vivo virulence of two strains of P. tularensis was duplicated. As a consequence of intracellular multiplication, normal macrophages were killed in relation to the virulence of the strain employed. Alveolar macrophages were also collected from immune rabits and macrophage mortality and bacterial growth were significantly suppressed below levels observed with normal macrophage preparations. The effect of immune serum couldonly be ascribed a minor role in the observed reactions. A marked intravenous toxicity of P. tularensis for the rabbit was observed with both virulent and attenuated strains. The toxicity was possessed only by viable preparations and could be elicited in animals immune to virulent challenge.

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Document Details

Document Type
Technical Report
Publication Date
May 01, 1966
Accession Number
AD0484223

Entities

People

  • John E. Nutter
  • Quentin N. Myrvik

Tags

Communities of Interest

  • Biomedical

DTIC Thesaurus Topics

  • Adaptive Immunity
  • Animals
  • Anti-Bacterial Agents
  • Bacteria
  • Biological Sciences
  • Blood
  • Cells
  • Chemistry
  • Immune Serums
  • Immune System
  • Immunity
  • Infection
  • Macrophages
  • Public Health
  • Rodents
  • Tissues
  • Vaccination

Fields of Study

  • Biology

Readers

  • Microbial Pathology
  • Toxicology/Environmental Toxicology