THE LOCALIZATION OF INHALED PARTICULATE MATERIAL

Abstract

This investigation tried various ways of cutting and staining sections from lungs of animals exposed to a variety of aerosols containing particulates. The most successful approach involved the use of polystyrene latex spheres (Dow Chemical Company). These particles could be seen with phase microscopy on frozen sections of lung tissue. Anchoring of extracellular particles and definitive localization of particles remain problems as yet not solved by any of the modifications of standardized technics tried. Counting of particles in sections indicated that particles are diminished in the lungs twelve days after exposure and are removed by thirty days post exposure. No accumulation of particles was observed in hilar lymph nodes, spleen, liver or kidney in animals subjected to single or multiple exposures.

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Document Details

Document Type
Technical Report
Publication Date
Apr 01, 1966
Accession Number
AD0486653

Entities

Organizations

  • West Virginia University

Tags

DTIC Thesaurus Topics

  • Animals
  • Biological Laboratories
  • Biological Staining And Labeling
  • Chemical Engineering
  • Dielectric Polymers
  • Dyes
  • Engineering
  • Laser Dyes
  • Lung
  • Lymph Nodes
  • Lymphatic System
  • Materials
  • Optical Equipment
  • Particles
  • Polystyrenes
  • Rodents
  • West Virginia

Readers

  • Aerosol Science/Aerosol Physics
  • Immunology
  • Systems Analysis and Design