STABILIZATION OF EEE VIRUS AGAINST ULTRAVIOLET AND IONIZING RADIATIONS
Abstract
It was reported previously that the protective effect of thiourea and related compounds on UV-irradiated eastern equine encephalitis (EEE) virus and the infectious RNA of this virus appeared to be specific for compounds containing the C+S group, and to be dependent both upon an interaction of the compound with the viral nucleic acid and upon the light-absorbing character of the compound. Further investigation has shown that the effect is more extensive than was reported. Yeast RNA, tested early in this study, was completely without effect. The subsequent finding that compounds not containing a C=S group, e.g., 6-nitro 1 H-benzotriazole, retarded the UV inactivation of EEE virus led to the determination that the purines and pyrimidines, and apparently the nucleosides and nucleotides, all exert a protective effect against UV. Also, compounds that protected EEE virus against UV inactivation were effective as well against ionizing radiation. However, known radioprotective compounds, such as cysteamine, which also protected EEE virus against ionizing radiation, were completely ineffective against UV radiation.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1966
- Accession Number
- AD0487270
Entities
People
- Jo Ann F. Comer
- Ralph F. Wachter