GENE TRANSFER BY CONJUGATION BETWEEN P. PSEUDOTUBERCULOSIS F-LAC AND P. PESTIS
Abstract
Introduction of the F-lac episome from Escherichia coli into Pasteurella pseudotuberculosis yielded a strain of P. pseudotuberculosis F-lac that could transfer its genes by conjugation to Pasteurella pestis. The selected P. pseudotuberculosis marker arginine+ was transferred at frequencies between 1/ 1,000,000 and 1/10,000,000 per donor cell. Unselected donor characters such as melibiose of rhamnose fermentation, phage sensitivity, and ureas production appeared at different frequencies in the recombinants. The transferred markers were unrelated to the transfer of F-lac or its subsequent loss from the recipient cell.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jul 01, 1966
- Accession Number
- AD0487274
Entities
People
- Brian C. Morris
- Thomas W. Burrows
- William D. Lawton