An Investigation into the Use of Immobilised Cholinesterase for the Automatic Detection of Nerve Agents

Abstract

The automatic detection of nerve agent vapors using cholinesterase covalently bonded to a modified polymethacrylate ion exchange resin and incorporated into an absorbent permeable paper, is described. The system responds to G-agent concentrations as low as 0.005 mg/cum in under 5 minutes and to high agent concentrations in 5 - 8 seconds. The electrochemical method used to monitor the inhibition of enzymic activity has been studied and the problems associated with electrode design and the ability to achieve a stable operating baseline are discussed. A rapid photometric method for the assay of insoluble cholinesterase is described.

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Document Details

Document Type
Technical Report
Publication Date
May 01, 1971
Accession Number
AD0595689

Entities

People

  • C. Stratford
  • E. J. Olsen
  • G. Williams
  • James H. Lambert

Tags

Communities of Interest

  • Ground and Sea Platforms

DTIC Thesaurus Topics

  • Chemical Synthesis
  • Chemistry
  • Detection
  • Detectors
  • Electrochemical Cells
  • Electrodes
  • Enzyme Inhibitors
  • Flow Rate
  • G Agents
  • Governments
  • Hydrolysis
  • Ion Exchange
  • Ion Exchange Resins
  • Measurement
  • National Security
  • Nerve Agents
  • Polymers

Readers

  • Analytical Chemistry
  • Neurotoxicology