ALTERATIONS IN GLYCOLYSIS BY CELL-FREE RAT BRAIN HOMOGENATE UNDER HIGH OXYGEN PRESSURE.
Abstract
Glycolysis in cell-free rat brain homogenate was studied by lactate production under anaerobic and aerobic conditions, and at high pressures of oxygen (HOP), usually 5 atm. absolute. Glycolysis was markedly and rapidly increased at high oxygen pressure, more so with phosphate buffer alone than with combined phosphate and bicarbonate buffers. The HOP effect mimicked a Pasteur-like effect in this system and also resembled the effects of sodium azide and of potassium cyanide upon the system. This HOP response depended upon the total oxygen pressure, not the total mechanical gas pressure per se. Varying phosphate concentration had no significant effect on the response. Pyruvate also accumulated under HOP. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Dec 12, 1963
- Accession Number
- AD0603257
Entities
People
- E. M. Neptune Jr.
- H. C. Sudduth
- J. J. Thomas Jr.
Organizations
- Naval Medical Research Center