TRACHOMA VIRUS RESEARCH
Abstract
The dynamics of growth, immunological and chemical characterization of PLT agents were studied using cell culture, biochemical, immunological and radioisotope tools. This work was not confined to the PLT agents. PPLO and viruses such as influenza and arboviruses were also under investigation. An abstract of results of continuous passage of trachoma in HeLa cells has been published (Jenkin and O'Donnell, 1963). Recently 47 and 53 cell culture passage of trachoma strains, TW3 and Bour, respectively, showed continued pathogenicity in monkey eyes. The lack of second cycles of growth of trachoma in the HeLa cell system seems associated with interferon production. In this laboratory trachoma does not produce a CPE in HeLa cells permitting the use of Chikungunya, a group A arbovirus which does cause a CPE, as a challenge organism for detection of interferon. Lipid analysis of host (chick allantoic fluid - 'L' cells) and purified PLT organisms (6BC and meningopneumonitis virus) using thin layer and gas chromatography techniques showed quantitative and qualitative differences in the phospholipid fractions between the host and parasite as well as between parasite strains. Gas chromatography and thin layer chromatography analysis of fatty acids of phospholipid species of different strains of PLT is under investigation.
Document Details
- Document Type
- Technical Report
- Publication Date
- Oct 30, 1964
- Accession Number
- AD0608171
Entities
People
- H. M. Jenkin
Organizations
- University of Washington