GLYCOGEN: CHARACTERIZATION, BIOSYNTHESIS AND METABOLISM.
Abstract
In an attempt to obtain a product more closely approach ing the state of glycogen in intact animal tissues, a procedure for the extraction of this polysaccharide is described. This procedure is designed to avoid physical and chemical alteration by strong alkali, acid, or high temperature and distortion of the molecular weight spectrum resulting from selective losses during sedimentation. Glycogen obtained by means of the cold water procedure is stable in the presence of various agents that are known to disrupt hydrogen bonds and to disperse aggregates. The cold water procedure has no effect on the molecular size and distribution of alkali-extracted glycogen added to the tissue homogenate prior to the extraction. These and other observations discussed in this paper indicate that the product obtained by the cold water procedure approaches more closely the state of glycogen in the living cell than the products of the various extraction methods used hitherto.
Document Details
- Document Type
- Technical Report
- Publication Date
- Nov 01, 1964
- Accession Number
- AD0608674
Entities
People
- Ernest Bueding
- Stanley A. Orrell Jr.
Organizations
- Johns Hopkins University