MECHANISM OF PROTEIN SYNTHESIS.
Abstract
During the course of the past few years a cell-free system was developed from the hybrid yeast Saccharomycetes dobzyanskii x S. fragilis. With this system it was possible to examine a number of problems dealing with the mechanism of protein synthesis which are included in the final report. In addition, a companion program was initiated with S. lactis to (a) examine the similarities in cell-free incorporating systems from related organisms and (b) to facilitate the isolation of the transfer enzyme, a step towards a completely defined incorporating system. Cellfree incorporating systems are generally inefficient and have a short half-life. One of the major defects is usually attributed to nuclease action. To better understand, and control, nuclease action in cell extracts, a further characterization of these enzymes was undertaken. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 31, 1964
- Accession Number
- AD0608776
Entities
People
- Harlyn O. Halvorson
Organizations
- University of Wisconsin–Madison