ENZYMATIC STUDIES ON THERMOPHILIC BACTERIA

Abstract

The objectives of the research were (1) to prepare crystalline alpha-amylase from thermophilic bacteria and to study the physical and chemical properties of the preparations in order to obtain data relative to the problem of thermal stability; (2) to study the biosynthesis of alpha-amylase by Bacillus stearothermophilus; (3) to study conditions of induction of a lysogenic system in B. stearothermophilus; (4) to isolate and purify enzymes from thermophilic bacteria related to amino acid and carbohydrate metabolism; and (5) to study the physical and chemical properties of the above enzymes. Studies were initiated on the sequence of the alpha-amylase of B. stearothermophilus by using chains A and B which are formed by performic acid oxidation. Conditions for the isolation of peptides were examined and efforts were made to obtain sufficient material to study the sequence of the two chains.

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Document Details

Document Type
Technical Report
Publication Date
Dec 31, 1964
Accession Number
AD0609954

Entities

People

  • L. L. Campbell

Organizations

  • University of Illinois Urbana–Champaign

Tags

DTIC Thesaurus Topics

  • Acids
  • Amino Acids
  • Anabolism
  • Bacteria
  • Bacteriophages
  • Carbohydrate Metabolism
  • Carbohydrates
  • Chemical Properties
  • Deoxyribonucleic Acids
  • Enzymes
  • Glycerols
  • Materials
  • Metabolism
  • Microbiology
  • Sequences
  • Sugar Alcohols
  • Thermal Stability

Readers

  • Marksmanship and Weaponry.
  • Microbial Pathology
  • Molecular Genetics