SPECIFIC FRACTIONATION OF HUMAN ANTIDEXTRAN ANTIBODIES. III. FRACTIONATION OF ANTI-DEXTRAN BY SEQUENTIAL EXTRACTION WITH OLIGOSACCHARIDES OF INCREASING CHAIN LENGTH AND ATTEMPTS AT SUBFRACTIONATION,
Abstract
Elution of human antidextran absorbed on sephadex with oligosaccharides of increasing chain length yielded purified antibody fractions which vary in their binding affinity for oligosaccharides of the isomaltose series as measured by their capacities to inhibit complement fixation of the antibody with dextran. The data are interpreted as indicating that these antibody molecules are heterogeneous and that with one antiserum the bulk of such heterogeneity in complementarity occurs in the region from the first alpha-a-glucosyl linkage to the fourth glucose from the non-reducing end of the hapten; with another antiserum the fifth glucose was involved. Attempts at subfractionation to obtain more homogeneous fractions were relatively unsuccessful. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 25, 1964
- Accession Number
- AD0611959
Entities
People
- Elvin A. Kabat
- Justus Gelzer
Organizations
- Columbia University