A CAPILLARY TUBEI NILE BLUE METHEMOGLOBIN REDUCTION TEST FOR GLUCOSE-6-PHOSPHATE DEHYDROGENASE DEFICIENCY,
Abstract
A modification of the methemoglobin reduction test for G-6-PD deficiency is introduced which uses smaller amounts of blood in capillary tubes (approximately 0.025 ml), a shorter period of incubation, the substitution of Nile blue for methylene blue, and alteration of the concentrations of sodium nitrite and glucose in order to obtain the desired result. The test is based on the principle that in the presence of Nile blue, methemoglobin reduction is mainly NADP-linked and is thus dependent on G-6-PD activity (BEUTLER and BALUDA). In the presence of Nile blue, erythrocytes from unaffected subjects reduce nitrite-induced methemoglobin to oxyhemoglobin at a faster rate than do G-6-PD deficient erythrocytes.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1963
- Accession Number
- AD0613564
Entities
People
- F. A. Ajmar
- H. Frischer
- J. E. Bowman
- Miriam Kahn
- P. E. Carson
Organizations
- University of Chicago