COMPARISON OF THE PEPTIDE MAPS OF ANTI= BODIES AGAINST AN ACIDIC AND A BASIC DETERMINANT GROUP,
Abstract
Antibodies were isolated from the serum of rabbits injected with azophenylarsonate-BSA or azophenyltrimethylammonium-BSA. The structures of antibodies formed against the two haptens and also those formed against BSA were compared by fingerprinting. No reproducible differences could be detected in the peptide patterns of the univalent fragments obtained by the papain digestion of the antibodies from pooled immune sera against each of the two antigens. When the two hapten-specific antibody species were each prepared from single antisera, a difference involving a single peptide spot was observed. The same difference was found in the peptide maps of the BSA-specific antibody fragments. This suggests that the small difference between the fingerprints in the haptenspecific antibodies may arise from genetic differences in the globulin pattern of the two rabbits or from differences in the organ distribution of the two antigens rather than from the different immunological specificity of the two haptens. A small portion of the peptides obtained by tryptic digestion of the antibodies of their univalent fragments does not migrate upon electrophoresis of chromatography but remains at the origin. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 13, 1963
- Accession Number
- AD0614218
Entities
People
- Felix Haurowitz
- James L. Groff
Organizations
- Indiana University Bloomington