ROLE OF THE KIDNEY IN THE FORMATION OF ACTIVE POLYPEPTIDES IN THE BLOOD.
Abstract
Contents: Purification and chemical identification of anephrotensin obtained from nephrectomized dogs; Purification and pharmacological studies of anephrotensin obtained from chicken serum and from the frog, Calliptocephelella Gayi serum; Studies on Anephrotensin obtained from the serum of hypertensive and normal rats; Comparative studies on electrolytes and water urinary excretion between hypertensive and normotensive rats; and effects of growth hormone on the renal excretion of water and electrolytes induced by renin. Conclusions: Nephrectomized dog anephrotensin (partially purified) has at least 3 components: (a) polypeptide vasoconstrictor; (b) a second vasoconstrictor (anephrotensin); and (c) polypeptide vasodilator (bradykinin). A procedure was developed for high degree purification. Some mol.wt. studies and amino acid analyses were performed. Anephrotensin was isolated from the chicken and frog and preliminary tests in rats showed stimulatory action on adrenal glands. An improved process for purification of rat anephrotensin permitted comparison of activities with that from other sources. Hypertension was induced by encapsulating kidney. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 15, 1965
- Accession Number
- AD0615574
Entities
People
- G. Swaneck
- Hector Croxatto
- J. Roblero