METHEMOGLOBIN REDUCTION IN INTACT G6PD DEFICIENT AND HBM ERYTHROCYTES,
Abstract
A microscopic elution procedure has been described by KLEIHAUER and BETKE (Nature, 199:1196(1963)), capable of differentiating erythrocytes containing oxidized hemoglobin (which appear as ghosts) from those containing oxyhemoglobin (red). Previous studies using this technique in glucose-6-phosphate dehydrogenase (G6PD) deficient and HbM erythrocytes have been presented in Clin. Res., XII: 86 (1964). Parallel genetic, enzymatic, spectro-photometric and cell elution studies are shown: (1) to clarify the conditions for interpretation, (2) to study intact erythrocytes of different genetic variants of G6PD deficiency and, (3) to investigate further some of the problems associated with the expression of this X-linked gene in erythrocytes of female heterozygotes. In addition, data on 4 variants of HbM will be given where pertinent. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1964
- Accession Number
- AD0615622
Entities
People
- H. Frischer
- J. E. Bowman
- P. E. Carson
Organizations
- University of Chicago