THE STEADY-STATE SYSTEM IN IMMUNE HEMOLYSIS DESCRIPTION AND ANALYSIS; APPLICATION TO THE ENUMERATION OF SAC'4,
Abstract
The occurrence of a steady state in the reaction between EAC'4, C'1a and C'2 has been demonstrated. This steady state is characterized by a constant concentration of SAC'1a,4,2a+SAC'4,2a and a slow linear decrease in C'2 concentration. A model for this system, which is based on the assumptions that C'1a combines reversibly with SAC'4 and that the formation of SAC'1a,4,2a from SAC'1a,4 conforms to enzyme kinetics, accounts for all the observed properties of the reaction. Detailed analysis of the system indicates that it provides a method for the enumeration of SAC'4 in a preparation of EAC'4. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 01, 1964
- Accession Number
- AD0616201
Entities
People
- Ann T. Mckenzie
- Louis G. Hoffmann
- Manfred M. Mayer
Organizations
- Johns Hopkins University