SELECTIVE INACTIVATION OF FIBRIN STABILIZING FACTOR CONTAMINANT IN FIBRINOGEN,
Abstract
The finding that mercaptide-forming agents inactivate Fibrin Stabilizing Factor (FSF) present in fluoresceinlabelled human or bovine fibrinogen preparations led to the development of a combined procedure in which both FSF inactivation and fibrinogen labelling with fluorescein are carried out simultaneously. This combined procedure makes possible the rapid and reproducible preparation of a labelled fibrinogen product suitable for use in an assay system for the quantitative measurement of FSF activity. It is the purpose of this paper to describe: (a) the procedure used for the preparation of such tagged, treated fibrinogen; (b) some of the characteristics of the latter; (c) its application to the assay of FSF activity.
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1962
- Accession Number
- AD0617590
Entities
People
- J. L. Koppel
- John H. Olwin
- Stevenson Swigert