DETECTION OF TISSUE SPECIFIC ANTIGENS ON CELL SURFACES.
Abstract
Antibody sensitization and complement dependent properties of IgG (7S) IgM (19S) rabbit anti-horse kidney (Forssman) antibodies were studied in systems using sheep erythrocytes and dog kidney tissue culture cells. Agglutination, indirect antiglobulin, and mixed antiglobulin techniques were used to determine the extent of antibody sensitization of cells. Immune adherence and mixed conglutination were used to demonstrate the presence of sites of complement fixation on these sensitized cells. The relationship between the presence of fixed complement and subsequent lysis of the test cells was examined. Guinea pig complement appeared to be less well fixed by IgG than IgM antibodies. However the complement fixed by IgG antibody was shown to be highly lytic both for sheep erythrocytes and dog kidney tissue culture cells. The fixation of complement by IgM antibodies resulted in weak and relatively ineffective cytolysis of dog kidney cells while retaining its lytic activity on sensitised sheep erythrocytes. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Jun 15, 1965
- Accession Number
- AD0617690
Entities
People
- K. W. Sell
Organizations
- University of Cambridge