CHARACTERISTICS OF THE ATP-ASE ACTIVITY OF ISOLATED NEURONS OF RABBIT,
Abstract
Isolated and homogenised DEITERS' neurons from the lateral vestibular nucleus of rabbit in a Krebs-Ringer solution containing no added Mg(++), 1.3 micro moles/ml and 5 micro moles/ml Mg(++) broke down ATP at the maximal rate of 0.29 = 0.20, 2.40 = 0.20, and 5.95 = 0.63 micro micro moles/cell/hr. In 1.3 mM Mg(++) solution the single cell homogenates took up phosphate at the mean rate of 2.6 = 0.2 micro micro moles/cell/hr. If the rabbits were injected 1 hour before with 20 mg/kg body weight of 2-amino-1-propene-1,1,3, tricarbonitrile (triap), the breakdown of ATP in these latter media was 0.82 = 0.44, 2.5 = 0.60, and 6.7 = 1.1 micro micro moles/cell/hr, respectively, and the quantity of inorganic phosphate liberated did not decrease. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Aug 31, 1964
- Accession Number
- AD0617980
Entities
People
- H. Hillman
- H. Hyden
Organizations
- University of Gothenburg