THE EFFECTS OF INSULIN UPON GLUCOSE METABOLISM AND PROTEIN SYNTHESIS BY THE ANTERIOR PITUITARY IN VITRO,
Abstract
The effects of insulin upon the metabolism of glucose by single rat anterior pituitary glands in vitro were compared with the effects of raising the concentration of glucose in the suspending medium. The pattern of stimulation with insulin differed from that induced by increasing glucose. Insulin enhanced glucose oxidation, glycogenesis and lipogenesis out of proportion to the increase in glucose uptake. The anterior pituitary exhibited relative insensitivity to insulin in vitro compared to muscle and adipose tissue. The incorporation of glycine C14 into tissue protein was stimulated by insulin, and this effect was undiminished in the absence of glucose in the medium. The incorporation of C14alpha-amino-isobutyric acid from the medium into the anterior pituitary was greater than that reported for rat diaphragm and was not affected by insulin. The data indicate that insulin could potentially affect hormonogenesis in anterior pituitary through stimulation of protein synthesis. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1964
- Accession Number
- AD0618506
Entities
People
- Charles J. Goodner
Organizations
- University of Washington