ISOLATION OF COAGULANT AND ANTICOAGULANT PRINCIPLES FROM THE VENOM OF TRIMERESURUS GRAMINEUS,
Abstract
The venom of Trimeresurus gramineus was separated into five fractions, namely alpha, beta, gamma, delta, and epsilon by means of zone electrophoresis on starch with borate-NaCl buffer; pH 7.6, and ionic strength 0.062. Only the alpha-fraction migrated towards the anode. The beta-fraction remained at the position of application and the other three fractions migrated towards the cathode. The highest thrombin-like and TAMe-esterase activities were located next to the peak of the alpha-fraction on the anode side. Both activities were about seven times stronger than those of the crude venom. The proteolytic activity of the alpha-fraction was less than one half that of the crude venom. The beta-fraction showed only feeble thrombinlike, TAMe-esterase and proteolytic activities. The gamma-fraction was the principal portion with anticoagulant as well as proteolytic activities. The delta and epsilon-fractions had little effect on blood coagulation, although a moderate proteolytic activity was present in the delta-fraction. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Jan 01, 1965
- Accession Number
- AD0620629
Entities
People
- Chaoho Ouyang
- Shoei-yn Shiau
Organizations
- National Taiwan University