ZONE ELECTROPHORESIS OF HUMAN PAROTID SALIVA IN ACRYLAMIDE GEL,
Abstract
The report concerns an examination of acrylamide gel as another medium for the zone electrophoresis of parotid saliva proteins. Preliminary experiments with acrylamide-gel strips yielded protein patterns in which twenty or more fractions could be visually distinguished when the electrophoresis was carried out in 0.1 M tris-EDTAboric acid buffer at pH 9.0. Under the conditions of the experiment, more than half the staining components migrated anodically with generally better resolution than is evident for the cathodically-migrating components. Salivary amylase, a troublesome factor in the starch-gel electrophoresis of saliva proteins, does not attack acrylamide gel.
Document Details
- Document Type
- Technical Report
- Publication Date
- Mar 01, 1965
- Accession Number
- AD0620977
Entities
People
- B. L. Lamberts
- T. S. Meyer