INHIBITION OF GUINEA PIG C'2 BY RABBIT ANTIBODY. QUANTITATIVE MEASUREMENT OF INHIBITION, DISCRIMINATION BETWEEN IMMUNE INHIBITION AND COMPLEMENT FIXATION, SPECIFICITY OF INHIBITION AND DEMONSTRATION OF UPTAKE OF C'2 BY EAC'LA,4,
Abstract
On repeated inoculation with alum-precipitated, partially purified guinea pig C'2, rabbits form antibody which inhibits the hemolytic activity of C'2. A sensitive method has been designed for precise quantitative measurement of inhibiting anitbody. The procedure excludes the possibility of complement fixation and, hence, the inhibitory effect is attributed to steric hindrance. The shape of the anti-C'2 neutralization curve indicates that a single molecule of antibody suffices for inhibition of C'2, and that the C'2 molecule can bind several molecules of neutralizing antibody. Quantitative inhibition analyses indicate that C'2 physically combines with EAC'la,4 to yield the intermediate EAC'la,4,2a. (Author)
Document Details
- Document Type
- Technical Report
- Publication Date
- Sep 23, 1964
- Accession Number
- AD0621390
Entities
People
- Jimmie A. Miller
- Manfred M. Mayer
Organizations
- Johns Hopkins University