5-HYDROXYTRYPTAMINE RELEASE BY BRAIN SLICES.

Abstract

Brain slices from the rat were allowed to respire for 2 hr in a modified Tyrode's solution containing the monoamine oxidase inhibitor 1-phenyl-2-hydrazinopropane (alpha-methyl phenyl hydrazine, JB-516). The filtered Tyrode's solution was shown to contain 5-hydroxytryptamine (Serotonin, 5-HT) by both bioassay and fluorometric techniques. Preliminary evidence indicates that the amount of 5-HT in the filtrate is increased significantly by adding reserpine and JB-516 to the incubation medium. These findings suggest that a sensitive bioassay procedure, coupled with the use of brain slices. offers an approach for the study of factors affecting the storage and release of endogenous 5-HT. (Author)

Document Details

Document Type
Technical Report
Publication Date
Jun 01, 1965
Accession Number
AD0621528

Entities

People

  • Malcolm B. Bowers Jr.
  • Margaret G. Filbert
  • Stanford T. Roodman

Organizations

  • Edgewood Chemical Biological Center

Tags

DTIC Thesaurus Topics

  • Anatomy
  • Bioassay
  • Biochemistry
  • Biological Sciences
  • Brain
  • Central Nervous System
  • Chemical Compounds
  • Chemistry
  • Hydrazines
  • Incubation
  • Inhibitors
  • Nervous System
  • Reserpine
  • Serotonin

Readers

  • Analytical Chemistry
  • Cardiovascular Physiology